CHEMISTRY COLLEGE

A protein subunit from an enzyme is part of a research study and needs to be characterized. a total of 0.185 g of this subunit was dissolved in enough water to produce 2.00 ml of solution. at 28 ?c the osmotic pressure produced by the solution was 0.138 atm. what is the molar mass of the protein?

Answers

Answer 1
Answer:

Answer:The molar mass of the protein is 16,544 g/mol.

Explanation:

Volume of the solution ,V = 2.00 ml= 0.002 L

Osmotic pressure = 0.138 atm

temperature at which solution is prepared = 28° C = 301 K()

Number of moles of protein,n=

Osmotic pressure is determined by :

The molar mass of the protein is 16,544 g/mol.

Answer 2
Answer:

The molar mass of the protein : 16517.86 g/mol

Further explanation

Osmosis pressure is the minimum pressure given to the solution so that there is no osmotic displacement from a more dilute solution to a more concentrated solution.

General formula:

π = osmosis pressure (atm)

M = solution concentration (mol / l)

R = constant = 0.08205 L atm mol-1 K-1

T = Temperature (Kelvin)

Mass of a protein: 0.185 g

Volume: 2.00 ml of solution. at 28 C

the osmotic pressure: 0.138 atm

We enter into the equation to find moles from protein concentrations

π = M. R. T

t = 28 C + 273 = 301 K

0.138 = M. 0.08205. 301 K

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